Reporter

Part:BBa_K4799604:Design

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


pNtNRAMP5_GUS_T-Nos


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1027
    Illegal SpeI site found at 1391
    Illegal SpeI site found at 1921
    Illegal SpeI site found at 2240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4049
    Illegal SpeI site found at 1027
    Illegal SpeI site found at 1391
    Illegal SpeI site found at 1921
    Illegal SpeI site found at 2240
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2031
    Illegal BamHI site found at 2014
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1027
    Illegal SpeI site found at 1391
    Illegal SpeI site found at 1921
    Illegal SpeI site found at 2240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1027
    Illegal SpeI site found at 1391
    Illegal SpeI site found at 1921
    Illegal SpeI site found at 2240
    Illegal NgoMIV site found at 2464
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1486
    Illegal BsaI.rc site found at 2264
    Illegal BsaI.rc site found at 2879
    Illegal SapI.rc site found at 3050


Design Notes

Test if the promoter will induce GUS express after cadmium treatment.



Source

plasmid

References